Comments |
Restriction digests of the clone give the following sizes (kb): PstI--4.0, 1.9, 1.0; BglI--3.0, 2.4, 1.2, 0.3; EcoRI--3.9, 3.0; SalI--6.9. Restriction digests of the clone give the following sizes (kb): PstI--4.0, 1.9, 1.0; BglI--3.0, 2.4, 1.2, 0.3; EcoRI--3.9, 3.0; SalI--6.9. The insert includes the following restriction sites (nt from the 5' end): PvuII--958, 1172, 2576, 2739, 2827, 2849, 3572, 3589; XbaI--1900, 2722, 3286; BamHI--448; SmaI--284; HindIII--3563. Contains the complete coding sequence, plus 5' (397 bp) and 3' (520 bp, including the stop codon) untranslated regions. Extends 196 bp beyond the reported sequence in the 3' direction. |
References |
Meyer R, et al. Cloning of the DNA-binding subunit of human nuclear factor kappaB: the level of its mRNA is strongly regulated by phorbol ester or tumor necrosis factor alpha. Proc. Natl. Acad. Sci. USA 88: 966-970, 1991. PubMed: 1992489
Claus Scheidereit, personal communication
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