產(chǎn)品名稱 |
Human rhinovirus 48 |
商品貨號(hào) |
B162831 |
Classification |
Picornaviridae, Enterovirus, Rhinovirus B |
Deposited As |
Rhinovirus 48 |
Agent |
Human rhinovirus 48 |
Strain |
1505 |
Applications |
Respiratory research |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1.0 mL per vial |
Storage Conditions |
-70°C or colder |
Images |
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Comments |
ATCC® VR-1875™ was derived from ATCC® VR-515™ following treatment with diethyl ether to remove mycoplasma contamination.
Recognized Reference Strain: Collaborative Report on Rhinoviruses, Nature (Lond.) 213: 761, 1967. |
Effect on Host |
CPE, refractile rounding and sloughing with cell degeneration |
Recommended Host |
WI-38 cells (ATCC® CCL-75™) |
Growth Conditions |
Temperature: 33°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 1.0). Adsorb 1.5-2.0 hours at 33°C in a humidified 5% CO2 atmosphere on a rocking platform. End adsorption by adding virus growth medium.
Incubation: 3-5 days at 33°C in a humidified 5% CO2 atmosphere on a rocking platform, until CPE is progressed through 80% of the monolayer. |
Name of Depositor |
VV Hamparian, RM Conant, Ohio State University |
References |
Kapikian A, et al. Characterization of two newly recognized rhinovirus serotypes of human origin. Proc. Soc. Exp. Biol. Med. 122: 1155-1162, 1966. PubMed: 4288216
Kapikian AZ et al. Rhinoviruses: a numbering system. Nature 213: 761-762, 1967. PubMed: 4291698
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