| 產(chǎn)品名稱 |
West Nile virus |
| 商品貨號(hào) |
B162943 |
| Classification |
Flaviviridae, Flavivirus |
| Agent |
West Nile virus |
| Strain |
Monoclonal antibody to West Nile virus Envelope Protein, Clone E58 (produced in vitro) |
| Common Name |
West Nile virus |
| Applications |
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E58 hybridoma supernatant by protein G affinity chromatography. The antibody is reported to be strongly neutralizing and reactive using flow cytometry and Western blot analysis. Emerging infectious disease research |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E58 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E58 antibody is reported to bind to domain III in the envelope glycoprotein. |
| Product Format |
frozen Each vial of VR-1614 contains approximately 100 µL of purified monoclonal antibody in PBS. |
| Storage Conditions |
?Store at -20°C or colder immediately upon arrival Repeated freeze-thaw cycles should be avoided |
| Comments |
Antibody Class: IgG2aκ Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E58 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E58 antibody is reported to bind to domain III in the envelope glycoprotein. Functional activity is confirmed by reactivity in immunofluorescence assays using Vero cells (ATCC CCL-81) infected with WNV and by ELISA using WNV-infected cell lysates [WNV, Eg101 (Egypt 1951)]. The antibody is reported to be strongly neutralizing and reactive using flow cytometry and Western blot analysis. |
| Effect on Host |
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E58 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E58 antibody is reported to bind to domain III in the envelope glycoprotein. |
| Name of Depositor |
MS Diamond |
| References |
Oliphant T, et al. Development of a Humanized Monoclonal Antibody with Therapeutic Potential Against West Nile Virus. Nat. Med. 11: 522-530, 2005. PubMed:15852016.
Oliphant T, et al. Antibody Recognition and Neutralization Determinants on Domains I and II of West Nile Virus Envelope Protein. J. Virol. 80:12149-12159, 2006. PubMed:17035317.
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