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Construct size (kb): 50.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Charon 4A
Intact vector size: 46.000
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
promoter: none
replicon: lambda
terminator: none
Cross references:

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GC
Genomic copy number: unique
Gene name: group-specific component (vitamin D binding protein)
Contains complete coding sequence?: N
Chromosome: 4; Localization: 4 q12-q13
Tissue: fetal liver (ATCC 37333)
Type of DNA: genomic
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert size (kb): 19.1
Cross references: DNA Seq. Acc.: L10641
Nucleotides ~1-19100 of the insert correspond to
nucleotides ~29850-49180 of L10641.

Insert lengths(kb): 19.10000038146973

Tissue: fetal liver (ATCC 37333)

Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) [GC]

Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 11, 7.2, 4.3, 3.3, 2.7, 1.6; HindIII--10.5, 9.55, 8.8, 5.75, 4.35, 4.1, 1.5, 1.4, 0.9, 0.6, 0.3; BamHI--13, 8, 6.6, 6.2, 3.9 (doublet), 3, 2.3, 1.5, 0.5;

Restriction digests of the clone give the following sizes (kb): BglII--14, 9.2, 5.8, 5, 4.9, 2.7, 2.2, 1.9, 1.8, 1.35, 1, 0.65, 0.35; SstI--18, 11, 4.6, 4.2, 3.85, 2.4, 1.95, 1.65, 1.2.

The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--1.6, 4.3, 8.6, 11.9; HindIII--0.4, 1.5, 2.9, 3.8, 4.2, 13.75, 18.1; SacI--14.6, 18.8.

Begins in intron 6 and extends into the 3' flanking region.

ATCC 95666/95667 and 95668/95669 overlap by 7.8 kb and share an EcoRI fragment of 2.7 kb.

ATCC 95668/95669 and 95670/95671 overlap by 11.9 kb and share an EcoRI fragment of 3.3 kb.

Contains an Alu repeats, and part of a THE repeat.