Comments |
Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 7.211, 6.549 (vector), 5.352, 4.859, 3.774, 2.022, 1.766, 1.666, 1.335, 1.331, 1.048, 0.940, 0.830, 0.790, 0.790, 0.726, 0.625, 0.590, 0.589, 0.545, 0.417, 0.391, 0.380 (vector). IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 108805-109878 of the sequence of chromosome VIII. |
References |
Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229
Linda Riles, personal communication
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