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Construct size (kb): 4.360000133514404

DNA: cDNA

Insert lengths(kb): 1.1599999666214

Tissue: L cell

Gene product: thymidine kinase 1, soluble [Tk1]

Insert contains MspI (2), Sau3AI (2), TaqI, HaeIII (7), ClaI, and AvaI (3) sites (not including the polylinker).

Restriction digests of the clone give the following sizes (kb): BamHI--3.2, 1.5; SalI--4.7; PstI/HindIII--3.2, 0.86, 0.5.

Contains a functional cDNA for cytoplasmic thymidine kinase which is close to full length.

pMTK4 detects genomic HindIII fragments of 23 kb and 4.7 kb in C57BL/10J, C3H/HeJ, A/HeJ, and BALB/cJ mice. It also detects 9.3 kb and 3.7 kb fragments also present in Ltk- cells, suggesting the latter are not functional cytosolic Tk genes.