For plasmid transformations, use patches from frozen cultures rather than single colonies to avoid undesired mutations
transformation host
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Type Strain
no
Genotype
MATa ura3-52 leu2delta1 trp1delta63 msh6::deltahisG
RefAlani E. The Saccharomyces cerevisiae Msh2 and Msh6 proteins form a complex that specifically binds to duplex oligonucleotides containing mismatched DNA base pairs. Mol. Cell. Biol. 16: 5604-5615, 1996. PubMed: 8816473
Preceptrol®
no
Mating Type
a
Ploidy
haploid
Comments
For plasmid transformations, use patches from frozen cultures rather than single colonies to avoid undesired mutations
transformation host
Medium
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 25.0°C
Name of Depositor
E Alani
Special Collection
NCRR Contract
Isolation
derived from ATCC 90840
References
Alani E. The Saccharomyces cerevisiae Msh2 and Msh6 proteins form a complex that specifically binds to duplex oligonucleotides containing mismatched DNA base pairs. Mol. Cell. Biol. 16: 5604-5615, 1996. PubMed: 8816473
Alani E. The Saccharomyces cerevisiae Msh2 and Msh6 proteins form a complex that specifically binds to duplex oligonucleotides containing mismatched DNA base pairs. Mol. Cell. Biol. 16: 5604-5615, 1996. PubMed: 8816473