Restriction digests of the clone give the following sizes (kb): BamHI--3.9; BamHI/EcoRI--3.0, 1.0; BglI--3.9; BglI/PstI--2.5, 1.5; BglI/PvuII--2.8, 1.2. The insert contains the following restriction sites (nt from the 5' end): BalI--267; XmnI--696. Contains a constitutively spliced exon encoding amino acids 1057-1117, the following intron, and an alternatively spliced exon which contains 2 internal donor sites. |